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786o ccrcc cancer cell line  (ATCC)


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    ATCC 786o ccrcc cancer cell line
    PBMCs from <t>ccRCC</t> patients confer resistance to cabozantinib treatment. (A–E) Schematic of the experimental set-up. microDUO, high throughput 384-well open microchannel multi-culture plates (Onexio Biosystems) were used to determine the effect of ccRCC PBMCs on angiogenesis. (F) The total length of the endothelial tubes was normalized to EC + DMSO-only control condition (dotted line).
    786o Ccrcc Cancer Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2275 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/786o+ccrcc+cancer+cell+line/786-O/pmc08730366-47-0-8
    Average 99 stars, based on 2275 article reviews
    786o ccrcc cancer cell line - by Bioz Stars, 2026-10
    99/100 stars

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    1) Product Images from "Immune cell mediated cabozantinib resistance for patients with renal cell carcinoma"

    Article Title: Immune cell mediated cabozantinib resistance for patients with renal cell carcinoma

    Journal: Integrative Biology

    doi: 10.1093/intbio/zyab018

    PBMCs from ccRCC patients confer resistance to cabozantinib treatment. (A–E) Schematic of the experimental set-up. microDUO, high throughput 384-well open microchannel multi-culture plates (Onexio Biosystems) were used to determine the effect of ccRCC PBMCs on angiogenesis. (F) The total length of the endothelial tubes was normalized to EC + DMSO-only control condition (dotted line).
    Figure Legend Snippet: PBMCs from ccRCC patients confer resistance to cabozantinib treatment. (A–E) Schematic of the experimental set-up. microDUO, high throughput 384-well open microchannel multi-culture plates (Onexio Biosystems) were used to determine the effect of ccRCC PBMCs on angiogenesis. (F) The total length of the endothelial tubes was normalized to EC + DMSO-only control condition (dotted line).

    Techniques Used: High Throughput Screening Assay, Control

    PBMCs from patients with ccRCC upregulated secretion of pro-angiogenic factors in response to cabozantinib treatment. (A) The concentration of pro-angiogenic factors was quantified using a multiplex magnetic bead-based assay (R&D Systems). The heatmap was used to visualize the differential regulation of pro-angiogenic factors in response to cabozantinib treatment in the absence and presence of cancer cells and ccRCC PBMCs. (B) The concentration of pro-angiogenic cytokines and growth factors obtained from conditioned media from mono-culture, co-culture, and tri-culture conditions treated with cabozantinib. Bars represent average ± SD of n = 3 wells for EC + DMSO, EC + cabozantinib, EC + 786O + cabozantinib, and n = 9 wells (three wells per patient, three patients in total) ( * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.005, and # P ≤ 0.0001).
    Figure Legend Snippet: PBMCs from patients with ccRCC upregulated secretion of pro-angiogenic factors in response to cabozantinib treatment. (A) The concentration of pro-angiogenic factors was quantified using a multiplex magnetic bead-based assay (R&D Systems). The heatmap was used to visualize the differential regulation of pro-angiogenic factors in response to cabozantinib treatment in the absence and presence of cancer cells and ccRCC PBMCs. (B) The concentration of pro-angiogenic cytokines and growth factors obtained from conditioned media from mono-culture, co-culture, and tri-culture conditions treated with cabozantinib. Bars represent average ± SD of n = 3 wells for EC + DMSO, EC + cabozantinib, EC + 786O + cabozantinib, and n = 9 wells (three wells per patient, three patients in total) ( * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.005, and # P ≤ 0.0001).

    Techniques Used: Concentration Assay, Multiplex Assay, Bead-based Assay, Co-Culture Assay

    PLSR analysis of MDSC and T cell subsets and cabozantinib resistance. PBMCs isolated from nine patients with ccRCC undergoing systemic therapy were profiled for MDSC and T cell subtypes using flow cytometry. A PLSR model was generated using flow cytometry data as independent variables and cabozantinib resistance quantified by total tube length from tri-culture angiogenesis assay as a dependent variable. (A) Scores plot separated patients according to the degree of resistance measured in the tri-culture model along the principal component 1 (PC1). (B) Loadings plot shows covariance among the immune cell subtypes with the cabozantinib resistance. (C) VIP of immune cell subtypes with VIP score >1. (D) Changes in Th9, Th17, Th22, and Th2 cells for four patients who are receiving cabozantinib treatment (solid lines) were compared to five patients who are undergoing other therapies (dotted lines, three patients for nivolumab and two patients for pazopanib). (E) Immune gene-signature analysis of 530 patients with ccRCC from TCGA cohort showed that patients with higher expressions of the Th22 gene signature had significantly better overall survival (log-rank test P = 0.027). There was no significant correlation between the Th9 gene signature and overall survival.
    Figure Legend Snippet: PLSR analysis of MDSC and T cell subsets and cabozantinib resistance. PBMCs isolated from nine patients with ccRCC undergoing systemic therapy were profiled for MDSC and T cell subtypes using flow cytometry. A PLSR model was generated using flow cytometry data as independent variables and cabozantinib resistance quantified by total tube length from tri-culture angiogenesis assay as a dependent variable. (A) Scores plot separated patients according to the degree of resistance measured in the tri-culture model along the principal component 1 (PC1). (B) Loadings plot shows covariance among the immune cell subtypes with the cabozantinib resistance. (C) VIP of immune cell subtypes with VIP score >1. (D) Changes in Th9, Th17, Th22, and Th2 cells for four patients who are receiving cabozantinib treatment (solid lines) were compared to five patients who are undergoing other therapies (dotted lines, three patients for nivolumab and two patients for pazopanib). (E) Immune gene-signature analysis of 530 patients with ccRCC from TCGA cohort showed that patients with higher expressions of the Th22 gene signature had significantly better overall survival (log-rank test P = 0.027). There was no significant correlation between the Th9 gene signature and overall survival.

    Techniques Used: Isolation, Flow Cytometry, Generated, Angiogenesis Assay

    Related Articles

    Cell Culture:

    Article Title: Immune cell mediated cabozantinib resistance for patients with renal cell carcinoma
    Article Snippet: .. 786O ccRCC cancer cell line was purchased from ATCC (ATCC, Cat. CRL-1932TM) and were cultured in RPMI-1640 (ThermoFisher Scientific, Cat. 11875119) media supplemented with 10% fetal bovine serum (VWR) and 100 U/ml penicillin/streptomycin (ThermoFisher Scientific). ..



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    786o ccrcc cancer cell line  (ATCC)
    99
    ATCC 786o ccrcc cancer cell line
    PBMCs from <t>ccRCC</t> patients confer resistance to cabozantinib treatment. (A–E) Schematic of the experimental set-up. microDUO, high throughput 384-well open microchannel multi-culture plates (Onexio Biosystems) were used to determine the effect of ccRCC PBMCs on angiogenesis. (F) The total length of the endothelial tubes was normalized to EC + DMSO-only control condition (dotted line).
    786o Ccrcc Cancer Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/786o+ccrcc+cancer+cell+line/786-O/pmc08730366-47-0-8
    Average 99 stars, based on 1 article reviews
    786o ccrcc cancer cell line - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    PBMCs from ccRCC patients confer resistance to cabozantinib treatment. (A–E) Schematic of the experimental set-up. microDUO, high throughput 384-well open microchannel multi-culture plates (Onexio Biosystems) were used to determine the effect of ccRCC PBMCs on angiogenesis. (F) The total length of the endothelial tubes was normalized to EC + DMSO-only control condition (dotted line).

    Journal: Integrative Biology

    Article Title: Immune cell mediated cabozantinib resistance for patients with renal cell carcinoma

    doi: 10.1093/intbio/zyab018

    Figure Lengend Snippet: PBMCs from ccRCC patients confer resistance to cabozantinib treatment. (A–E) Schematic of the experimental set-up. microDUO, high throughput 384-well open microchannel multi-culture plates (Onexio Biosystems) were used to determine the effect of ccRCC PBMCs on angiogenesis. (F) The total length of the endothelial tubes was normalized to EC + DMSO-only control condition (dotted line).

    Article Snippet: 786O ccRCC cancer cell line was purchased from ATCC (ATCC, Cat. CRL-1932TM) and were cultured in RPMI-1640 (ThermoFisher Scientific, Cat. 11875119) media supplemented with 10% fetal bovine serum (VWR) and 100 U/ml penicillin/streptomycin (ThermoFisher Scientific).

    Techniques: High Throughput Screening Assay, Control

    PBMCs from patients with ccRCC upregulated secretion of pro-angiogenic factors in response to cabozantinib treatment. (A) The concentration of pro-angiogenic factors was quantified using a multiplex magnetic bead-based assay (R&D Systems). The heatmap was used to visualize the differential regulation of pro-angiogenic factors in response to cabozantinib treatment in the absence and presence of cancer cells and ccRCC PBMCs. (B) The concentration of pro-angiogenic cytokines and growth factors obtained from conditioned media from mono-culture, co-culture, and tri-culture conditions treated with cabozantinib. Bars represent average ± SD of n = 3 wells for EC + DMSO, EC + cabozantinib, EC + 786O + cabozantinib, and n = 9 wells (three wells per patient, three patients in total) ( * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.005, and # P ≤ 0.0001).

    Journal: Integrative Biology

    Article Title: Immune cell mediated cabozantinib resistance for patients with renal cell carcinoma

    doi: 10.1093/intbio/zyab018

    Figure Lengend Snippet: PBMCs from patients with ccRCC upregulated secretion of pro-angiogenic factors in response to cabozantinib treatment. (A) The concentration of pro-angiogenic factors was quantified using a multiplex magnetic bead-based assay (R&D Systems). The heatmap was used to visualize the differential regulation of pro-angiogenic factors in response to cabozantinib treatment in the absence and presence of cancer cells and ccRCC PBMCs. (B) The concentration of pro-angiogenic cytokines and growth factors obtained from conditioned media from mono-culture, co-culture, and tri-culture conditions treated with cabozantinib. Bars represent average ± SD of n = 3 wells for EC + DMSO, EC + cabozantinib, EC + 786O + cabozantinib, and n = 9 wells (three wells per patient, three patients in total) ( * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.005, and # P ≤ 0.0001).

    Article Snippet: 786O ccRCC cancer cell line was purchased from ATCC (ATCC, Cat. CRL-1932TM) and were cultured in RPMI-1640 (ThermoFisher Scientific, Cat. 11875119) media supplemented with 10% fetal bovine serum (VWR) and 100 U/ml penicillin/streptomycin (ThermoFisher Scientific).

    Techniques: Concentration Assay, Multiplex Assay, Bead-based Assay, Co-Culture Assay

    PLSR analysis of MDSC and T cell subsets and cabozantinib resistance. PBMCs isolated from nine patients with ccRCC undergoing systemic therapy were profiled for MDSC and T cell subtypes using flow cytometry. A PLSR model was generated using flow cytometry data as independent variables and cabozantinib resistance quantified by total tube length from tri-culture angiogenesis assay as a dependent variable. (A) Scores plot separated patients according to the degree of resistance measured in the tri-culture model along the principal component 1 (PC1). (B) Loadings plot shows covariance among the immune cell subtypes with the cabozantinib resistance. (C) VIP of immune cell subtypes with VIP score >1. (D) Changes in Th9, Th17, Th22, and Th2 cells for four patients who are receiving cabozantinib treatment (solid lines) were compared to five patients who are undergoing other therapies (dotted lines, three patients for nivolumab and two patients for pazopanib). (E) Immune gene-signature analysis of 530 patients with ccRCC from TCGA cohort showed that patients with higher expressions of the Th22 gene signature had significantly better overall survival (log-rank test P = 0.027). There was no significant correlation between the Th9 gene signature and overall survival.

    Journal: Integrative Biology

    Article Title: Immune cell mediated cabozantinib resistance for patients with renal cell carcinoma

    doi: 10.1093/intbio/zyab018

    Figure Lengend Snippet: PLSR analysis of MDSC and T cell subsets and cabozantinib resistance. PBMCs isolated from nine patients with ccRCC undergoing systemic therapy were profiled for MDSC and T cell subtypes using flow cytometry. A PLSR model was generated using flow cytometry data as independent variables and cabozantinib resistance quantified by total tube length from tri-culture angiogenesis assay as a dependent variable. (A) Scores plot separated patients according to the degree of resistance measured in the tri-culture model along the principal component 1 (PC1). (B) Loadings plot shows covariance among the immune cell subtypes with the cabozantinib resistance. (C) VIP of immune cell subtypes with VIP score >1. (D) Changes in Th9, Th17, Th22, and Th2 cells for four patients who are receiving cabozantinib treatment (solid lines) were compared to five patients who are undergoing other therapies (dotted lines, three patients for nivolumab and two patients for pazopanib). (E) Immune gene-signature analysis of 530 patients with ccRCC from TCGA cohort showed that patients with higher expressions of the Th22 gene signature had significantly better overall survival (log-rank test P = 0.027). There was no significant correlation between the Th9 gene signature and overall survival.

    Article Snippet: 786O ccRCC cancer cell line was purchased from ATCC (ATCC, Cat. CRL-1932TM) and were cultured in RPMI-1640 (ThermoFisher Scientific, Cat. 11875119) media supplemented with 10% fetal bovine serum (VWR) and 100 U/ml penicillin/streptomycin (ThermoFisher Scientific).

    Techniques: Isolation, Flow Cytometry, Generated, Angiogenesis Assay